Ultrafiltration Technology rau Kev Tshem Tawm Endotoxin hauv Kev Siv Tshuaj Tiv Thaiv Kab Mob Rabies: Ib qho Scalable thiab Nqis -Tshuaj purification zoo

Taw qhia

Cov txheej txheem tshem tawm endotoxin ib txwm muaj, xws li chromatography, feem ntau koom nrog cov txheej txheem nyuaj, cov nqi khiav haujlwm siab, thiab ncua sijhawm ua haujlwm. Hnub no,Ultrafiltration technologytau dhau los ua ib qho kev nyiam tshaj plaws hauv kev tshuaj tiv thaiv purification vim tias nws ua rau muaj kev sib xyaw ua ke thiab kev tshem tawm endotoxin thaum tswj hwm cov tshuaj tiv thaiv kab mob siab.

Kab lus no tshawb txog kev siv tswv yim ntawm a300 kDa PES ultrafiltration cassettenyob rau hauv kev tsim tshuaj tiv thaiv kab mob vwm thiab tshuaj xyuas nws cov kev ua tau zoo hauv kev txo qis endotoxin, antigen rov qab, thiab loj - ua kom muaj peev xwm tsim tau.

 

Vim li cas Endotoxin Control Matters hauv Kev Tsim Tshuaj Tiv Thaiv

Endotoxins yog cov kab mob ruaj khov heev uas tuaj yeem ua haujlwm txawm tias tom qab kho cua sov. Thaum lub sij hawm tsim cov tshuaj tiv thaiv, kev kis kab mob tuaj yeem tshwm sim ntawm ntau theem ntawm kev ua dej ntws, kab lis kev cai ntawm tes, lossis kev sau qoob loo.

Rau cov tshuaj tiv thaiv kab mob xws li tshuaj tiv thaiv kab mob vwm, tswj cov qib endotoxin ntawm tus kab mob sau qoob muaj ntau yam zoo:

Txo cov dej purification hauv qab

Txhim kho cov khoom zoo kawg nkaus

Minimizes batch tso tawm txaus ntshai

Enhances manufacturing efficiency

Xyuas kom ua raws li cov kev cai tswj hwm

Raws li kev siv tshuab sib cais membrane txuas ntxiv mus,Ultrafiltration systemstau dhau los ua ib qho kev daws teeb meem rau ob qho tib si kab mob concentration thiab endotoxin tshem tawm.

 

Cov ntaub ntawv thiab cov txheej txheem

Kab mob thiab kab mob

Tus kab mob Rabies tas li: CTN-1V

Host cell: Vero cells

Ultrafiltration Membrane

Membrane Khoom: PES (Polyethersulfone)

Molecular Nyhav Txiav-Tawm (MWCO): 300 kDa

Membrane cheeb tsam: 2.5 m²

Khoom siv: Guidling 300 kDa Ultrafiltration Cassette (G25000300K)

Kev daws tsis haum

0.01 M PAB

0.15 M NaCl

pH 7.6

Endotoxin Detection Method

Endotoxin qib ua ntej thiab tom qab ultrafiltration tau txiav txim siab siv covLimulus Amebocyte Lysate (LAL) kev soj ntsuam, uas tseem yog tus qauv kev lag luam rau kev kuaj kab mob endotoxin.

 

Rabies Virus Harvest Preparation

Cov txheej txheem sau tus kab mob suav nrog:

Kauj Ruam 1: Cell Culture

Vero hlwb tau rov ua dua thiab coj los ntawm 37℃kom txog thaum ua tiav monolayer.

Kauj Ruam 2: Kev Tiv Thaiv Kab Mob

Cells tau kis tus kab mob CTN-1V rabies kab mob nyob rau ntawm qhov teev inoculation concentration.

Kauj Ruam 3: Kev Tshaj Tawm Kab Mob

Cov kab lis kev cai nruab nrab tau hloov nrog kev saib xyuas nruab nrab uas muaj 0.1% tib neeg cov ntshav albumin, tom qab ntawd incubation ntawm 32-33 degree.

Kauj Ruam 4: Kev Sau Kab Mob

Tus kab mob- muaj cov kua kab lis kev cai tau sau ntau zaus thiab sib sau ua ke los tsim cov tshuaj pub rau kev ua ultrafiltration.

 

Kev ntsuas qhov tseem ceeb Ultrafiltration Parameters rau Endotoxin tshem tawm

Peb qhov txheej txheem tseem ceeb tau tshawb xyuas:

1.Operating Pressure2.Concentration Factor3.Diafiltration Volume

Ntau cov khoom tsim tau raug sim los ntsuas lawv cov teebmeem ntawm endotoxin tshem tawm efficiency thiab antigen rov qab.

 

Cov nyhuv ntawm Kev Ua Haujlwm Siab

Plaub lub zog ua haujlwm tau raug tshuaj xyuas:

5psi ua

10psi ua

15psi ua

20psi ua

Kev sim tej yam kev mob:

30 × concentration factor

10 diavolumes ntawm buffer exchange

Cov txiaj ntsig

Txoj kev tshawb no qhia tau hais tias kev khiav hauj lwm siab muaj kev cuam tshuam tsawg kawg nkaus rau ob qho tib si endotoxin tshem tawm efficiency thiab antigen rov qab.

Qhov kev tshawb pom no muab cov neeg tsim khoom hloov pauv thaum xaiv cov kev ua haujlwm siab raws li cov khoom siv tshwj xeeb thiab cov kev xav tau ntau lawm.

 

Cov nyhuv ntawm Concentration Factor

Plaub yam concentration tau tshawb xyuas:

30×

50×

80×

100×

Kev sim tej yam kev mob:

Tsau siab: 20 psi

10 diavolumes ntawm buffer exchange

Cov txiaj ntsig

Raws li kev xav tau nce ntxiv, qib endotoxin hauv cov concentration tau nce me ntsis.

Txawm li cas los xij:

Antigen rov zoo nyob ruaj khov

80 × concentration tau pom zoo endotoxin txo

100 × concentration muab cov khoom tsim tau ntau dua thiab cov nqi tsim khoom qis dua

Cov txiaj ntsig no qhia tau tias ob qho tib si 80 × thiab 100 × cov ntsiab lus tseem ceeb yog tsim nyog rau kev lag luam - teev cov tshuaj tiv thaiv.

 

Cov nyhuv ntawm Diafiltration Volume (Feem ntau qhov tseem ceeb Parameter)

Diafiltration volumes soj ntsuam:

10 DV

15 DV

20 DV

25 DV

Kev sim tej yam kev mob:

100 × concentration

20 psi ua haujlwm siab

Cov txiaj ntsig

Lub diafiltration ntim tau pom tias yog qhov cuam tshuam tshaj plaws cuam tshuam rau kev tshem tawm endotoxin.

Raws li buffer exchange volume nce:

Endotoxin qib tau txo qis

Antigen rov zoo tseem nyob tsis tu ncua

Ntawm25 diafiltration ntim, endotoxin tshem tawm mus txog rau98.7%, thaum tuav cov tshuaj antigen zoo heev.

 

Cov ntsiab lus ua haujlwm

Parameter	Kev tshem tawm Endotoxin (%)	Antigen rov qab (%)
5psi ua	Mus txog 85.0	96.6
10psi ua	Txog rau 86.0	96.4
15psi ua	Mus txog 85.0	97.0
20psi ua	Mus txog 87.0	96.9
30 × Qhov siab	53.3–95.3	97.6
50 × Qhov siab	64.0–96.4	97.1
80 × Qhov siab	68.7–96.9	96.3
100 × Qhov siab	65.0–96.5	96.5
10 DV Diafiltration	62.5–96.3	97.6
15 DV Diafiltration	68.7–96.9	96.6
20 DV Diafiltration	80.0–98.0	97.1
25 DV Diafiltration	87.5–98.7	96.2

---

 

Cov kev tshawb pom tseem ceeb

Txoj kev tshawb fawb tau lees paub tias a300 kDa PES ultrafiltration membrane cassettemuaj txiaj ntsig zoo rau kev tshem tawm endotoxin hauv kev tsim tshuaj tiv thaiv kab mob vwm.

Cov ntsiab lus tseem ceeb suav nrog:

Diafiltration ntim yog cov txheej txheem tseem ceeb tshaj plaws.

25 diavolumes muab qhov siab tshaj plaws endotoxin txo.

Endotoxin tshem tawm tuaj yeem ncav cuag 98.7%.

Antigen rov zoo li qub tshaj 96%.

Ultrafiltration enables simultaneous concentration thiab endotoxin tshem tawm.

Cov txheej txheem yog tsim nyog rau ob qho tib si tsav- nplai thiab kev lag luam- nplai tsim tshuaj tiv thaiv.

 

Qhov zoo ntawm Ultrafiltration Over Traditional Chromatography

Piv nrog cov pa chromatography-raws li txoj kev tshem tawm endotoxin, ultrafiltration muaj cov txiaj ntsig tseem ceeb:

Tus nqi tsim khoom qis

Txo kev siv cov resin thiab cov kauj ruam ua kom yooj yim pab txo cov nuj nqis ua haujlwm.

Kev ua haujlwm siab dua

Concentration thiab endotoxin tshem tawm yog ua nyob rau hauv ib qho kev ua haujlwm.

Zoo heev Antigen Recovery

High rov qab tus nqi txo cov khoom poob thiab txhim kho tag nrho cov txheej txheem tawm los.

Ib qho yooj yim Scale-Up

Cov txheej txheem tuaj yeem hloov kho tau yooj yim los ntawm kev tsim kho hauv chav kuaj mus rau kev tsim khoom lag luam.

Nruam Manufacturing Compatibility

Ultrafiltration systems ua ke zoo nrog niaj hnub nruam bioprocessing platforms.

 

Xaus

Raws li cov tuam txhab tshuaj tiv thaiv txuas ntxiv mus nrhiav kev tsim khoom ntau dua thiab cov qauv nruj dua,Ultrafiltration technology rau endotoxin tshem tawmyog tshwm sim raws li ib tug nyiam purification lub tswv yim.

Kev thov ntawm a300 kDa PES ultrafiltration cassetteHauv kev tsim cov tshuaj tiv thaiv kab mob dev vwm no qhia tau tias muaj txiaj ntsig zoo ntawm endotoxin txo, kev rov ua kom muaj antigen siab, thiab kev ua kom zoo tshaj plaws. Los ntawm kev sib koom ua ke ultrafiltration thaum lub sij hawm tus kab mob sau qoob loo, cov tuam txhab lag luam tuaj yeem txo qis endotoxin lub nra nce mus, ua kom yooj yim hauv qab dej huv, thiab txhim kho kev tsim tshuaj tiv thaiv tag nrho.

Rau cov tshuaj tiv thaiv kab mob dev vwm, cov tshuaj tiv thaiv kab mob tsis ua haujlwm, thiab lwm yam khoom siv lom neeg, ultrafiltration muab pov thawj, ntsuas tau, thiab tus nqi - siv tau zoo rau kev tswj hwm endotoxin thiab kev ua kom zoo dua.